CytoFLEX nano Flow Cytometer

Beckman Coulter Life Sciences has introduced the new CytoFLEX nano Flow Cytometer, a device specifically designed for the analysis of extracellular vesicles (EVs) and nanoparticles.

Extracellular vesicles (EVs) are membrane-bound particles secreted by cells into the extracellular environment. They play a crucial role in communication, influencing physiological and pathological processes such as immune response, tissue regeneration, tumor growth, and more.

Analyzing EVs is extremely important for:

• Understanding mechanisms of signal transmission between cells.
• Studying biomarkers for early disease diagnosis.
• Developing new therapeutic approaches for treating complex pathologies.

Traditional flow cytometry has limited ability to detect EVs smaller than 100 nm. Additional methods like Nanoparticle Tracking Analysis (NTA) and Dynamic Light Scattering (DLS) somewhat improve the level of detail, but they often require multiple platforms, making the processes labor-intensive and complicating standardization.

What Makes CytoFLEX nano Unique?

Built on the same principles as the CytoFLEX platform, the CytoFLEX nano combines the capabilities of various methods into a single platform, ensuring the detection of nanoparticles as small as 40 nm, analysis of individual EV markers even with low expression (<10 antigen copies), and the ability to provide standardized, fast, and accurate analysis without using multiple systems.

Key Advantages and Features

• Sensitivity and Multi-Channel Detection
  6 fluorescent channels and 5 side scatter channels allow detailed analysis of even the smallest particles.
  Increased laser power and specially designed optical excitation paths collect more light, enhancing the efficiency of the analysis.
• Data Purity and Contamination Minimization
  Automated cleaning tools quickly detect and eliminate contamination, minimizing the risk of sample loss to <1%.
  The daily quality control process detects noise from nano- and micro-bubbles and triggers automatic purging and cleaning.
• Optimized Optical and Fluidic Systems
  Reduced sample and sheath fluid flow rates enable working with micro-samples, ensuring high accuracy of results.
  Sample collection with a small-volume piston pump ensures stability and accuracy of counting (>90%).
  Specialized sheath fluid, combined with a variable 5-nm filter, minimizes background noise.
• Ease of Use
  The intuitive CytExpert software allows easy system setup and adaptation to the needs of users at any level.
  Process automation reduces time and simplifies sample preparation.

Applications

The CytoFLEX nano opens new opportunities for researchers in the following areas:

• Medicine: Biomarker research, diagnostic test development, and innovative therapies.
• Pharmaceuticals: EV analysis for target identification and drug efficacy evaluation.
• Agrigenomics: Studying nanoparticles in plant systems to enhance crop yield and resilience.

CytoFLEX Nano will become your reliable assistant in research. This tool simplifies complex workflows, reduces the number of necessary analysis methods, and ensures confidence in your results. Compact and elegant, it fits perfectly into your workspace, creating the feeling that it has always been part of your laboratory.

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CytoFLEX nano Brochure