Nextera XT DNA Library Preparation Kit
The Nextera XT DNA Library Preparation Kit offers a fast and easy workflow for preparing, quantifying, and normalizing libraries for sequencing small genomes, PCR amplicons, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA from a low amount of input DNA (1 ng), making this kit amenable for use with precious samples available in limited quantity.
Key features
- Provides rapid library preparation in as little as 90 min with only 15 min of hands-on time
- Eliminates library quantification before sample pooling and sequencing
- Delivers quick results in under 8 hr using Illumina sequencing systems
- Achieves high, even coverage and accurate calls for PCR amplicons
- Produces high-quality de novo assembly of small genomes
- Allows scalability by processing and uniquely barcoding up to 384 samples in a single experiment
Specifications
Properties
Value
Properties:
Assay time
Value:
~5.5 hr from DNA extraction to normalized library. (Library prep time: ~90 minutes).
Properties:
Automation capability
Value:
Liquid handling robot(s)
Properties:
Hands-on time
Value:
15 min
Properties:
Input quantity
Value:
1 ng DNA
Properties:
Instruments
Value:
MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiniSeq System, NextSeq 500 System
Properties:
Mechanism of action
Value:
Enzymatic fragmentation
Properties:
Method
Value:
Amplicon sequencing, 16s rRNA sequencing, De novo sequencing, Whole-genome sequencing, Shotgun sequencing
Properties:
Multiplexing
Value:
Up to 384 uniquely indexed samples may be pooled and sequenced together
Properties:
Nucleic acid type
Value:
DNA
Properties:
Specialized sample types
Value:
Not FFPE-compatible, Low-input samples, Single cells
Properties:
Species category
Value:
Compatible with any species
Properties:
Target insert size
Value:
300 bp–1.5 kb
Properties:
Technology
Value:
Sequencing
Properties:
Variant class
Value:
Single nucleotide polymorphisms (SNPs), Structural variants
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